11/30/2022 0 Comments Siberian mouse torrent blowjobSARS-CoV-2 belongs to the Betacoronavirus genus of the Coronaviridae family, along with two other closely related highly pathogenic viruses, SARS-CoV and Middle East respiratory syndrome coronavirus (MERS-CoV). In the absence of protective immunity in the whole human population ( 4 ), SARS-CoV-2 has exhibited an unprecedented human-to-human transmission capability.Īlthough several vaccine candidates are being currently tested in clinical trials, no commercial COVID-19 vaccine is presently available. The pandemic of coronavirus disease 2019 (COVID-19) caused by the newly emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global health crisis ( 1 3 ). Thus, this mouse-adapted strain and associated challenge model should be of value in evaluating vaccines and antivirals against SARS-CoV-2. The protective efficacy of a recombinant RBD vaccine candidate was validated by using this model. The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation.ĭeep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. We adapted a clinical isolate of SARS-CoV-2 by serial passaging in the respiratory tract of aged BALBc mice. Serbian Mouse Torrent Serial Passaging In The new mouse model was used to study neutralizing antibodies and a vaccine candidate against the virus.Ībstract The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Meters45 m-45 for 1st meters41 torrent 008.īriefly, 9-month-old BALBc mice were intranasally inoculated with 7.2 10 5 plaque forming units (PFU) of SARS-CoV-2, and the lung tissues were collected from each passage for viral RNA load analysis at 3 days after inoculation.Įmail: (S.J.) (S.S.) qincfatbmi.ac.cn (C.-F.Q.) These authors contributed equally to this work.Īdaptation of this viral strain in the mouse appeared to be dependent on a critical amino acid change, Asn 501 to Tyr (N501Y), within the receptor-binding domain of the viral spike protein. Serbian Mouse Torrent Serial Passaging In.
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